Figure 4. ¹³C labeling of B. mori larvae as a pilot application in an invertebrate animal model.

(a) Fourth instar silkworm larvae were reared on ¹³C-labeled diets for 12 days through ecdysis to the fifth instar stage, resulting in the gradual assimilation of ¹³C-labeled metabolites. Two bars per sample stage are shown. (b) Correlation matrix of 56 commonly selected metabolites used for the analysis of metabolites detected during the 12 days of the feeding experiment. Positively correlated metabolites are in red and negatively correlated metabolites are in blue. The metabolites are sorted by their loadings on PC1. (c) Loadings of the metabolites on the PC1 axis.