Table 1.

Proposed oligomericity of Cry toxins

Oligomericity	Toxin	Experimental approach	Dimensions	Reference
Dimer	Cry1Ab	Incubating toxins with susceptible larval BBMVs1, analyzed by modified SDS-PAGE and Western blotting	∼ 130 kDa	[35]
Dimer & Trimer	Cry1Ac		∼ 130 kDa & ∼ 210 kDa
Dimer & Trimer	Cry1Ac	Using dynamics light scattering in buffer with different ionic strengths	Molecular mass of each oligomer was derived from hydrodynamic radius of proteins from dynamics light scattering	[36]
Trimer	Cry1Aa, Cry1Ab Cry1Ac	Incubating toxins with susceptible larval BBMVs1, analyzed by modified SDS-PAGE and Western blotting	∼ 200 kDa	[37,38]
Trimer	Cry4Ba	Incubating toxins with liposomes, analyzed by modified SDS-PAGE and Western blotting	∼ 200 kDa	[30]
Tetramer	Cry1Aa	AFM2 of toxin inserted in bilayers in liquid cell	Diameter 5 nm, each subunit 1.4 nm, depression 1.5 nm	[21]
Tetramer	Cry4Ba	AFM2 of toxin inserted in bilayers in liquid cell	Diameter 20-30 nm, height 2-4 nm	[20]
Tetramer	Cry1Ab	ScFV73 cross-linked toxin3, binding with ANS 4	∼ 250 kDa	[19]
Large multimer	Cry1Aa Cry1Ac Cry1Ca Cry1D Cry3Aa	Analyzed by native and modified SDS-PAGE, and size-exclusion chromatography	>600 kDa	[39]

¹BBMVs = Brush-border membrane vesicles

²AFM = Atomic force microscopy

³ScFV73 = A single chain antibody mimicking Bt-R1 receptor

⁴ANS = 8-Anilino-1-naphthalenesulfonate