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. 2008 Nov 26;3(11):e3812. doi: 10.1371/journal.pone.0003812

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Jiang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Following transplantation of HSCs into Min mice, spontaneously developing intestinal tumors were examined for donor-derived (GFP+), Lyve-1⁺, CD45⁻, F4/80⁻ lymphatic endothelial cells. (a) Hematoxylin and eosin (H&E) staining of a tumor in a Min mouse. (b) High magnification of the boxed area in panel a demonstrating Lyve-1+ (red) lymphatic vessels within the tumor. (c) Merged image of a donor-derived (green), Lyve-1+ (red) lymphatic endothelial cell (arrow) and a donor-derived hematopoietic cell (arrow head). (d) The donor-derived hematopoietic cell expresses CD45 and/or F4/80 (magenta), whereas the donor-derived LEC does not. (e) Merged image of c and d (green+ blue+ magenta = white). (DAPI stained nuclei are blue in b–e. Scale bars: a: 500 µm; b: 200 µm, c–e: 5 µm)