Fig. 3.

In vivo effect of TNF-α dosage in normal skin (A) and LNCaP pro 5 tumor (B) grown in a dorsal skin fold chamber. Tissues were incubated with TNF-α for 4 hours prior to freezing. The minimum temperature required for complete cell death was measured at the edge of perfusion defect from the infra-red image taken during the treatment. Values presented are mean ± standard deviation from 5–8 animals in treated groups were statistically different thaneach group. All TNF- α untreated freeze alone groups (*, p < 0.01).