In vitro effect of using NF-κB inhibitor BAY 11-7085 (BAY) and caspase inhibitor Z-VAD-FMK (ZVAD) and on (A) LNCaP monolayers with freezing to −10°C, 5 min hold time, and (B) MVEC monolayers with freezing to −5°C, 0 min hold time. TNF-α treatment was at 1000 ng/ml for 4 hours prior to freezing. 100 μM ZVAD and 10 μM BAY were present in the medida until viability assay. Viability was assessed at 48 hours (LNCaP cells) or 24 hours (MVECs) after freeze/thaw (FT). Values presented are the means ± standard error mean from 3 independent experiments, and control indicates untreated cells. Significant effect of inhibitors compared with no inhibitor (*, p < 0.05). Significant effects of BAY compared with ZVAD+BAY, and ZVAD compared with ZVAD+BAY were shown in brackets (*, p < 0.05).