Figure 3.

Differential binding of pY proteins from SW620 cells to SH2 domains from 15 different signalling proteins. Top panel: 1 mg of TCL was precipitated with 50 μg of bead-immobilised GST-SH2 fusion protein, precipitates repeatedly washed with RIPA buffer and bound proteins analysed by anti-pY western blotting. Bottom panel: Coomassie blue staining of the affinity purified GST-SH2 domain fusion proteins used for the precipitations. Approximately 10 μg of each protein was loaded onto the gel to analyse protein purity and integrity.