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Flow chart describing the modified sChIP procedure. From Arabidopsis chromatin, the first ChIP was performed using antibodies that recognize either the DBD1 or DBD2 proteins, following the standard ChIP procedure. After washing and elution, the second ChIP was done using the antibody for either the DBD1 or DBD2 proteins on the products of the first ChIP. The amount of actin and GLABROUS3 (GL3) DNA present at each step (before and after ChIP) was evaluated using qPCR.
