Figure 6.
Ectopic dSTIM results in smaller wings due to increased apoptosis and affects vein and bristle differentiation. Patterning of the adult wild-type (wt) wing (A) and wings expressing dSTIMFL induced by various Gal4 drivers (B-K). Overexpression of dSTIMFL induced by 32B.Gal4 (D) and sd.Gal4 (E) result in wing margin defects seen as notching at the wing margin (brackets) and formation of ectopic veins (arrowheads). Overexpression of dSTIMFL driven by MS1096.Gal4 results in smaller wing size, with no apparent margin defects, but loss of the distal end of vein V (arrow) (B). Expression of two transgene copies of dSTIMFL driven by MS1096.Gal4 results in a further reduction in wing blade size (C). Loss of some mechanosensory bristles along the anterior wing margin (bracket) and multiple wing hairs (arrows) are evident at higher magnification (C'). Duplication of occasional campaniform sensilla (arrow) (G) and duplication of margin bristles are evident (arrows) (I) when two transgene copies of dSTIMFL are driven by sca.Gal4, that are not seen in wild-type wings (F, H). Staining of 3rd instar larval wing discs with acridine orange shows few positive cells in MS1096.Gal4 discs (J), and a high number of positively stained cells in wing discs expressing dSTIMFL induced by MS1096.Gal4 (K), indicating an increased rate of apoptosis.