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. 2008 Oct 8;8:174. doi: 10.1186/1471-2180-8-174

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Copyright © 2008 Gremel et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Northern analysis of transcription for analysis of light response of lim1. Transcription was analyzed in constant darkness (preculture, DD) and after illumination (1800 lux, 25 μmol photons m^-2s^-1) for 15, 30 or 45 minutes in the wild-type strain QM9414 and a strain lacking the PAS-domain of the light regulatory protein ENVOY (Schmoll et al., 2005). Strains were cultivated on conventional Mandels Andreotti minimal medium with 1% (w/v) glycerol as carbon source. 20 μg of total RNA were loaded per lane, a hybridization with 18S rRNA is given as a loading control.