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. Author manuscript; available in PMC: 2009 Aug 8.
Published in final edited form as: Mol Cell. 2008 Aug 8;31(3):313–323. doi: 10.1016/j.molcel.2008.05.021

Figure 4. Sgs1 induction in sgs1-mn mus81Δ cells.

Figure 4

A. Sgs1 protein expression from the estrogen-inducible promoter. Samples from 3XHA-sgs1-mn/3XHA-SGS1-IN mus81Δ (MJL3244) were displayed on Western blots and probed with anti-HA antisera. Uninduced (−ES; blue line); estradiol added at 4 hours (+ES; purple line). Sgs1 protein levels are plotted relative to levels at 0 hr, reflecting protein expressed from sgs1-mn, which is roughly equal to endogenous Sgs1 levels in wild type (Jessop et al., 2006).

B. Sgs1 induction prevents JM accumulation. JMs were detected as in Figure 2A in DNA from 3XHA-sgs1-mn/3XHA-SGS1-IN mus81Δ (MJL3244) sporulated without estradiol (−ES), or with estradiol added at 4 (+ES4). Total JM levels are plotted in comparison to those seen in wild type and sgs1-mn mus81Δ (MJL3181). Data from 4 independent experiments were used.

C. Sgs1 induction partially restores nuclear division. The fraction of cells completing at least one meiotic division were determined by DAPI staining of wild type and of 3XHA-sgs1-mn/3XHA-SGS1-IN mus81Δ sporulated without estradiol or with estradiol added at 4 hours.

D. Effect of Sgs1 induction on COs and NCOs. Recombinants were detected and quantified as in Figure 1E and Supplemental Figure 2. Frequencies (% of total lane signal) are averages of 7 and 8 hour samples from 4 independent experiments.

All plots report mean ± s. e. m.