FIG. 2.

ATMs in clusters in obese mice are MGL1⁻CCR2⁺ and MGL1⁺ ATMs are retained. Characterization of ATMs in high-fat diet (HFD)-fed C57Bl/6 mice. Caveolin staining (blue) demonstrates loss of membrane integrity in lipid droplets. Staining patterns were observed in at least four independent mice. Bar = 50 μm. A and B: Interstitial ATMs are retained with obesity and are uniformly MGL1⁺F4/80⁺CD11b⁺. CD11c (C) and TLR4 (D) are strongly expressed in MGL1⁻ ATMs in clusters. Isolectin binding (E) and CCR2 expression (F) are increased in ATM clusters and do not overlap with MGL1 expression. G: IL-10 expression is retained in interstitial ATMs and is downregulated in ATM clusters. H: Partial loss of caveolin staining in a dying adipocyte surrounded by a thin wall of MGL1⁻CD11c⁺ ATMs. I: Reduced numbers of ATM clusters in epididymal fat in high-fat diet Ccr2^−/− mice. ATM clusters in Ccr2^−/− mice express CD11c similar to obese Ccr2^+/+ mice. J: ATM clusters in Ccr2^−/− mice downregulate MGL1 expression. (Please see http://dx.doi.org/10.2337/db08-0872 for a high-quality digital representation of this figure.)