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Gene expression of Shh, Ret, Bmp-4, VEGF-A, and TNF-α on gestational day 11 in the control (N) and manifestly diabetic (MD) groups containing 29 and 32 embryos, respectively. Nonmalformed and malformed N and MD offspring are denoted Nn (28 embryos), MDn (20 embryos), Nm (1 embryo), and MDm (12 embryos), respectively. Data are means ± SE. *P < 0.05 vs. N and #P < 0.05 vs. MDn (ANOVA).
