. Author manuscript; available in PMC: 2009 Dec 1.

Published in final edited form as: Psychopharmacology (Berl). 2008 Aug 19;201(2):203–218. doi: 10.1007/s00213-008-1268-7

Table 1.

Tissue levels of serotonin, 5-HIAA and serotonin turnover rates (5-HIAA:serotonin) 30 min after 5-HTP (80 mg/kg) or its vehicle in SERT +/+, +/− and −/− mice.

Measurement (pg/mg tissue) Brain area	Drug condition						Two-way ANOVA
Measurement (pg/mg tissue) Brain area	Vehicle			5-HTP			Genotype		Drug condition		Interaction
	SERT +/+	SERT +/−	SERT −/−	SERT +/+	SERT +/−	SERT −/−	F_2,31	p-value	F_1,31	p-value	F_2,31	p-value
Serotonin
Brain stem	961 ± 22	976 ± 62	284 ± 54^####	2498 ± 252	2611 ± 205	1922 ± 265	57.59	< 0.0001	769.63	< 0.0001	0.33	NS
Frontal cortex	763 ± 55	811 ± 65	243 ± 14^####	1595 ± 214	1600 ± 197	1071 ± 56	70.22	< 0.0001	370.86	< 0.0001	0.12	NS
Hippocampus	780 ± 67	741 ± 92	247 ± 32^####	2024 ±515	2005 ± 288	1432 ± 223	17.59	< 0.0001	202.08	< 0.0001	0.07	NS
Hypothalamus	1799 ± 199	1790 ± 225	580 ± 144^####	5237 ± 628	5251 ± 446	3688 ± 322	56.88	< 0.0001	751.03	< 0.0001	0.87	NS
Striatum	715 ± 50	737 ± 106	307 ± 109^####	3505 ± 399	3771 ± 647	3579 ± 498	2.00	NS (0.15)	561.97	< 0.0001	1.15	NS
5-HIAA
Brain stem	884 ± 168	591 ± 162	508 ± 133	5292 ± 563	5645 ± 496	5070 ± 622	2.34	NS (0.11)	1165.88	< 0.0001	2.07	NS
Frontal cortex	385 ± 97	292 ± 84	221 ± 62	3209 ± 277	3132 ± 447	2713 ± 171	5.99	0.006	1153.74	< 0.0001	1.98	NS
Hippocampus	795 ± 191	512 ±129	618 ±534	4172 ± 351	4081 ± 384	3617 ± 331	3.33	0.049	839.53	< 0.0001	2.15	NS
Hypothalamus	999 ± 209	675 ± 233	600 ± 174	7236 ± 1055	7110 ± 746	6370 ± 571	3.43	0.045	966.50	< 0.0001	1.00	NS
Striatum	589 ± 223	483 ± 125	417 ± 171	5512 ±449	5713 ± 313	5269 ± 680	1.59	NS	1630.89	< 0.0001	0.9	NS
Serotonin turnover
Brain stem	92 ± 16	60 ± 14	178 ± 31^****	212 ± 17⁺⁺⁺⁺	216 ± 13⁺⁺⁺⁺	264 ± 11⁺⁺⁺⁺^****	73.12	< 0.0001	407.19	< 0.0001	11.63	< 0.0001
Frontal cortex	51 ± 13	36 ± 8	90 ± 22	203 ± 19	196 ± 12	254 ± 20	40.82	< 0.0001	869.76	< 0.0001	0.38	NS
Hippocampus	101 ± 20	68 ± 10	244 ± 194	214 ±42	208 ± 38	259 ± 50	6.28	0.005	10.25	0.003	1.85	NS
Hypothalamus	55 ± 9	37 ± 9	103 ± 17^****	138 ± 10⁺⁺⁺⁺	135 ± 5⁺⁺⁺⁺	173 ± 9⁺⁺⁺⁺^****	84.38	< 0.0001	588.16	< 0.0001	5.75	0.008
Striatum	83 ± 33	65 ± 11	134 ± 23^**	159 ± 20⁺⁺	155 ± 24⁺⁺⁺⁺	148 ± 17	6.18	0.006	65.32	< 0.0001	9.85	< 0.0001

Data represent the mean ± SEM. NS, not significant.

^####

p < 0.0001 compared to SERT +/+ mice in the same drug condition, based on pre-planned comparisons (Tukey post hoc tests) to confirm previous findings (Kim et al. 2005);

^**

p < 0.01

^****

p < 0.0001 compared to SERT +/+ mice in the same drug condition

⁺⁺

p < 0.01

⁺⁺⁺⁺

p < 0.0001 compared to vehicle-treated mice of the same genotype (Tukey post hoc tests for significant interactions).