Figure 5.

Expression of human β-globin chains in RBC of reconstituted mice and correlation between EGFP and human β-globin expression. (a) FACS analysis of RBC from a representative mouse 5 mo posttransplantation (Left) and a human control (Right), using a mAb specific for human β-globin. RBC from an unmanipulated mouse were used as a negative control. (b) Correlation between the proportion of RBC expressing EGFP and the proportion of RBC expressing human β-globin in reconstituted mice. The proportion of EGFP-positive and human β-globin-positive RBC was assessed separately by FACS between 1 and 5 mo posttransplantation. Each transplant recipient was analyzed twice; three recipients were analyzed three times.