Skip to main content
. 2008 Nov 12;416(Pt 2):179–187. doi: 10.1042/BJ20080328

Figure 4. HDAC1 presence in the inhibitory ERα transcriptional complex at the slug promoter and its regulation of slug expression.

Figure 4

(A) MDA-MB-468 clone 17, which overexpressed transfected ERα and showed significant slug inhibition with E2, was incubated in Phenol-Red-free DMEM supplemented with 5% DCC-FBS for 48 h, followed by 12 h of serum starvation. Cells were then incubated for 4 h with or without E2 (100 nM) and with or without an HDACI for 2 h. Total RNA was extracted and analysed for slug mRNA by real-time PCR. HDACI enhanced slug expression. Values are means±S.D. (B) MCF-7 cells were grown in Phenol-Red-free DMEM supplemented with 5% DCC-FBS for 48 h. After 12 h of serum starvation, the cells were either treated with vehicle ethanol or with 100 nM E2 for 4 h. ChIP assays were performed using antibodies directed against HDAC1, HDAC3 and HDAC4. E2 treatment led to the selective recruitment of HDAC1, but not HDAC3 or HDAC4, to the −456 to −68 region of the slug promoter. Input DNA was used to normalize the results.