Table 1.
Figure 1 panel | Detergent/EA Treatment | Total m/z matching BSA peptides ions* | Peptides common to a, b, & d |
---|---|---|---|
a) | No detergent, no EA | 30 | 25 |
b) | No detergent, EA extracted | 37 | 25 |
c) | 1% OG, no EA extraction | 0 | 0 |
d) | 1% OG, EA extracted | 34 | 25 |
e) | 0.5% NP-40, EA extracted | 26 | 17 |
f) | 1% Triton X-100, EA extracted | 23 | 17 |
g) | 1% SDS, EA extracted | 40 | 19 |
m/z between 700 and 4000
Data are derived from experiments depicted in Figure 1. The mass lists from the deisotoped peaks of the acquired m/z spectra were matched to BSA tryptic peptides with Expasy-FindPept (http://ca.expasy.org/tools/findpept.html). The number of predicted BSA tryptic peptides between 700 and 4000 m/z is 44.