Table 2003.13C.

Preparation of standard solutions (international laboratories)

		Alkaloid concentration, μg/mL
Volume stock standard solution, mL	Final volume, mL	EPa	PSa	ME	MP	NE	NP
0.50 [Level 1]	50	20.5	2.5	0.5	0.5	0.5	0.40
1.00 [Level 2]	50	41.0	4.9	1.0	1.0	1.0	0.80
1.50 [Level 3]	50	61.4	7.4	1.5	1.5	1.5	1.2
2.50 [Level 4]	50	102.4	12.3	2.5	2.5	2.5	2.0
3.50 [Level 5]	50	143.4	17.2	3.5	3.5	3.5	2.8
5.00 [Level 6]	50	204.8	24.6	5.0	5.0	5.0	4.0
Standard blankb	50	0.0	0.0	0.0	0.0	0.0	0.0

^aCorrected for HCl form.

^bBlank is 1 mL MeOH plus 99 mL diluent, E(c).