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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Vision Res. 2008 Sep 16;48(23-24):2425–2432. doi: 10.1016/j.visres.2008.07.016

Figure 7.

Figure 7

GC stimulation by wildtype versus mutant GCAP1. The effect of wildtype GCAP1 and GCAP1(N104K) on GC activity. A. The effect of increasing amounts of wildtype GCAP1 (●,■) and GCAP1(N104K) (○,□) on GC activity in washed membranes of HEK293 cells overexpressing GC1. The free Ca2+ concentration was 50 nM. B. The calcium titration of wildtype GCAP1- or GCAP1(N104K)-mediated GC1 activity. The GC1 activity was assayed in washed cell membranes of HEK293 cells overexpressing GC1 in the presence of 5 µM wildtype GCAP1 (●) or GCAP1(N104K) (○) and varying free Ca2+ concentrations set by Ca2+/EGTA buffering (Materials and Methods). The experiments were performed in triplicate. Error bars are standard deviation.