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. Author manuscript; available in PMC: 2008 Dec 6.
Published in final edited form as: Science. 2008 Jun 6;320(5881):1336–1341. doi: 10.1126/science.1157676

Figure 4. Tsix genetically interacts with Dcr.

Figure 4

(A) Allele-specific RT-PCR analysis. All RT- reactions were negative (not shown). Xist d0 samples (asterisks) were 10-fold overloaded to visualize low expression.

(B) Realtime RT-PCR of indicated transcripts, each normalized to β-actin.

(C) Immuno-RNA FISH for Xist and H3-3meK27 domains (arrowheads) on d10.

(D) Frequency of aberrant H3-3meK27 enrichment in the Xist+ subpopulation of indicated cell lines. n=100-150.

(E) Model: intersection of RNAi and XCI.

(F) Methylation-sensitive restriction analysis of the Xist promoter. Genomic DNA was digested with EcoRV or EcoRV+HpaII. % uncut (methylated) DNA at HpaII is plotted.