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. 2008 Nov 27;3(11):e3823. doi: 10.1371/journal.pone.0003823

Figure 4. Prdm14 is expressed most highly in ES cells.

Figure 4

4a) Northern analysis of embryonic tissue compared with the Evi32 tumor 27-186. Gapdh was used as a loading/RNA integrity control. We found expression of the 2.7 kb band in all ES cell samples, but none in any of the other embryonic tissues. The larger 6.5 kb transcript seen in the Evi32 tumor is not found in the ES cells. 4b and c) Quantitative RT-PCR was performed on cDNA obtained from the same tissue from 4a. Prdm14 and Oct-3/4 expression levels were quantified relative to 18S expression levels within each tissue. Expression levels are on a log scale to emphasize lower levels of expression. Results show specific expression of Prdm14 within Oct-3/4 positive ES-cells. All embryonic days are whole embryos unless otherwise indicated. YS = yolk sac.