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Respiratory uncoupling was determined from the kinetics of the proton leak in mitochondria isolated from MuPPARγKO (gray circles) vs. Flox control (black circles) mice. Oxygen consumption was measured as a function of mitochondrial inner membrane potential. Mitochondria were collected from gastrocnemius (n = 7–8) (A), myocardium (n = 6–7) (B), and kidney (n = 6–7) (C). **, P < 0.01 for global difference in the membrane potential vs. respiration curves between MuPPARγKO and Flox mice.
