Figure 4.

Effect of muscle PPARγ and ROSI treatment on AMPK levels, activity, and phosphorylation as measured in freshly isolated muscle (n = 4–5/genotype). Muscle lysates from MuPPARγKO (gray bars) and Flox (black bars) mice on normal-chow diet were immunoblotted for AMPKα1, AMPKα2, or phospho-ACC (pACC), with inset representative blots (A). AMPK activity was assayed in immunoprecipitates prepared using isoform-specific antibodies against AMPKα1 (B) or AMPKα2 (C) in muscle samples from MuPPARγKO (KO) and Flox mice on high-fat diet with (white bars) or without (gray bars) ROSI. Con Control. Likewise, phosphorylation of AMPK-Thr¹⁷² (D) and ACC (E) were determined in muscle lysates using phosphospecific antibodies. *, P < 0.05.