Figure 2.

A, LβT2 cells were transfected with 450 ng/well of the −126/+7 hFSHB-luc reporter overnight. Cells were serum starved overnight and then incubated for 30 min with the indicated inhibitors, followed by 10⁻⁷ m GnRH for 6 h. B, Cells were cotransfected with the reporter as in A plus 50 ng/well caMEKK1 or pcDNA3 (control). The following day, cells were treated with the inhibitors in serum-free medium for 24 h. In both panels, data represent the mean reporter activity from duplicate samples per treatment. In panels C and D, cells were transfected and treated as in B, except 400 (MKK6EE) or 500 ng/well (Raf-CAAX) expression vector was used, and treatments were performed in triplicate. Symbols show differences as assessed by post hoc analysis of the significant interactions.