Figure 6.

A, LβT2 cells were treated with 10⁻⁷ m GnRH1 for 2 h (lanes 4–6) after 30 min preincubation with 5 μm U0126 (lanes 2 and 5) or 25 μm SP600125 (lanes 3 and 6). Nuclear extracts were used in gel shifts with the −126/−94 probe as described (top panel, EMSA) or used in Western blot analyses and probed with the indicated antibodies. B, The analyses were performed as described in A, except cells were preincubated in 10 μm SB202190 for 30 min (lanes 5 and 6) or overnight (o/n) (lanes 3 and 4) before 10⁻⁷ m GnRH1 treatment for 2 h (lanes 2, 4, and 6).