Figure 8.

A, Cells were transfected in triplicate with the indicated hFSHB-luc reporters (450 ng/well) and FosB/cJun (100 ng each/well) expression vector(s). The data were analyzed by three-way ANOVA as described in Fig. 7A. B, Cells were transfected in triplicate with the −126/+7 hFSHB-luc reporter (450 ng/well), and the indicated Fos/Jun pairs (25 ng each/well) with and without A-Fos (200 ng/well). Cells were transfected with human FSHB (C) or LHB (D) reporters (450 ng/well) and the indicated concentrations of A-Fos. Cells were then treated with 10⁻⁷ m GnRH1 for 6 h before luciferase assays. Data in B–D were analyzed by two-way ANOVA and post hoc tests (Tukey) performed on the significant interactions. Symbols show differences as assessed by post hoc analysis.