Representative photomicrographs of immunohistochemistry showing kisspeptin-ir cells in the POA (A) and middle ARC (B) of the ewe brain. 3V, Third ventricle. Scale bar, 100 μm. C, Effect of season on kisspeptin expressing perikarya in the POA and ARC of the ewe. The number of kisspeptin-ir cells per section is shown for nonbreeding and breeding season ewes in the POA and rostral, middle, and caudal ARC. Values are means (± sem). *, P < 0.05 nonbreeding vs. breeding season.