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Effects of PRL and DEX on FoxO1 content, phosphorylation, and cellular distribution. INS-1 832/13 cells were grown in serum-containing medium and then incubated in serum free in the presence of rat PRL (40 nm), DEX (1 μm), a combination of the two, or diluent in basal medium for 15 min or 16 h. Phosphorylated FoxO1 (cyto phos), nuclear FoxO1, and tubulin were detected by Western blot, as described in Materials and Methods. Similar results were obtained in three experiments.
