Table 1.

Analysis of gene expression in INS-1 cells by quantitative real-time PCR

Gene	Accession no.	Primer sequence (top forward, bottom reverse, 5′–3′)	CT
Rat PGC1α	NM_031347	GCT GGA TGG CTT GGG ACA T	25
		CAA CCA GGG CAG CAC ACT CT
Rat PPARα	NM_013196	CCG TCG GGA TGT CAC ACA	24
		TTG CTT TCT CAG ATC TTG GCA TT
Rat PPARγ	AF246458	GGA TGT CTC ACA ATG CCA TCA G	29
		CGC CAA CAG CTT CTC CTT CT
Rat FoxO1	XM_001056726	TGT GCC CTA CTT CAA GGA TAA GG	22
		GTG GCG AAT TGA ATT CTT CCA
Rat UCP2	NM_019354	AAG ACC ATT GCA CGA GAG GAA	17
		TGG CAT TTC GGG CAA CAT
Rat CPT1	NM_031559	AAT TGC AGT GGT ATT TGA AGC TAA AA	22
		GAT ATA TTC TTC CCA CCA GTC ACT CA
Rat ADD1	AF286470	GCT ACC GTT CCT CTA TCA ATG ACA	20
		GCA AGA CAG CAG ATT TAT TCA GCT T
Rat Glut2	NM_012879	GCT TCC AGT ACA TTG CGG ACT T	17
		AGG ACC ACC CCA GCA AAA A
Rat riboprotein	X15096	CCC AGA GGT GCT GGA CAT CA	16
		GCG GAC ACC CTC TAG GAA GC

Values were obtained in cells grown in RPMI 1640 (11.1 mm glucose) supplemented with 10% FBS, 50 μm 2-mercaptoethanol, 1 mm sodium pyruvate, 10 mm HEPES, and 1% antibiotic/antimycotic solution.