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. Author manuscript; available in PMC: 2008 Nov 18.
Published in final edited form as: J Proteome Res. 2007 Jul 4;6(8):2993–3002. doi: 10.1021/pr060629m

Table 2.

List of selecteda secreted proteins observed across the MCF10 cell lines, documented by their position in the Mascot search list and the combined number of peptide matched in the combined search data b.

Protein Name NCBInr gi # MCF10A e MCF10AT MCF10 DCIS.com c MCF10CA cl. D
Mascot position Sequences matched Mascot position Sequences matched Mascot position Sequences matched Mascot position Sequences matched
Annexin 1 c 4502101 10 42 6 46 14 32 3 83
Annexin A2, isoform 2 c 4757756 3 63 3 80 3 114 2 134
Cathepsin D preprotein 4503143 88 13 21 47 9 66 19 93
Clusterin isoform 1 c 42716297 197 3 5 65
Cystatin B 4503117 70 9 169 4 151 4 79 24
Cystatin C precursor 4503107 25 22 25 21 87 17
Dystroglycan 1 precursor 4758116 189 1 96 5 39 16 90 11
Enolase 1 c 4503571 1 94 2 100 4 85 1 188
Galectin-3-binding protein (LGAL3BP) 5031863 76 18 2 133 7 75
Glutathione transferase c 4504183 4 34 4 42 19 22 23 40
H2A histone family, member E c 10800144 6 51 16 57 8 48 5 111
Lipocalin 2 38455402 6 76
Lysyl oxidase preproprotein (LOX) 20149540 118 4 116 7 332 2
Mesothelin isoform 1 preproprotein (MSLN) 53988378 46 24 77 16
Nucleophosmin 1 isoform 1 c 10835063 22 37 10 62 24 21 8 105
Peptidylprolyl isomerase isoform 1 (Cyclophilin A) 10863927 5 61 14 58 22 34 9 119
Peroxiredoxin 1 c 32455266 37 21 34 19 44 16 47 35
Plasminogen activator inhibitor type 1, member 2 24307907 167 9 161 10
Profilin 1 4826898 26 29 11 36 30 23 4 97
Quiescin Q6 isoform a 13325075 175 2 7 56 42 19
S100 calcium-binding protein A13 5174659 231 4 202 3
S100 calcium-binding protein A14 10190712 210 2 45 18 66 13
S100 calcium-binding protein A16 17933772 156 2 140 1 20 22 68 13
S100 calcium-binding protein A2 5174661 110 12 75 28 46 32
S100 calcium-binding protein A6 7657532 84 25 91 11 119 11 112 17
S100 calcium-binding protein P 5174663 16 21 170 6 113 12
Secreted frizzled-related protein 1 56117838 113 15 286 9
Secreted modular calcium-binding protein 1 isoform 2 11545873 13 29 45 27
Secreted protein, acidic, cysteine-rich (Osteonectin, SPARC) d 4507171 31 40 2
Serine (or cysteine) proteinase inhibitor, clade A (AAT) 50363217 12 55 81 14
Serine (or cysteine) proteinase inhibitor, clade B (ovalbumin) 4505789 238 5 213 3 231 13
Serpin peptidase inhibitor (AACT) 50659080 65 8 1 323 6 87
Syndecan 1 precursor 55749480 89 3 68 7 162 6
Thrombospondin 1 precursor 40317626 118 5 93 8 34 23 136 15
Tissue inhibitor of metalloproteinase 1 precursor 4507509 201 2 46 10 46 11 53 23
Transforming growth factor 4507467 176 10 1 176 126 1 41 34
Zinc alpha-2-glycoprotein 1 (ZAG) 4502337 24 28 94 5 61 8
a

Proteins listed were selected from over 200 from each proteome. The selection was based on their appearance high on the Mascot list and/or their significant change across cell lines.

b

Data from all search files from each secreted proteome (5 fractions each preparation, 3 preparations with 2 analyzed in duplicate) were combined and searched with Mascot against the RefSeq database from NCBI. The “most abundant” protein with the highest score is listed as Mascot position #1. The number of peptides include those that matched across all LC-MS/MS analyses. If a protein was not detected above the threshold score, a blank entry is used.

c

These proteins, while considered cytosolic, are predicted to be secreted by non-classical mechanisms using SecretomeP.

d

The SPARC in MCF10CA gave a Mascot score one point below the threshold and thus did not receive a position. It did have two peptides, one of which was manually sequenced and uniquely identified the protein.

e

The second most abundant protein in MCF10A and fifth in MCF10 DCIS.com was a keratin. This was consistently present in the three preparations of these cell lines.