. Author manuscript; available in PMC: 2008 Nov 18.

Published in final edited form as: Biochemistry. 2007 May 27;46(25):7536–7548. doi: 10.1021/bi700123s

Table 1.

Reactivity Patterns of Oxidant Probes

Oxidant:^a	Probe:
	DCHF-beads	HE^b	PPH	CPH/CMH	Amplex® Red
O₂^·-	-	+	+	+	-
H₂O₂	-	-	-	-	-
H₂O₂/HRP	+	+	+	+	+
H₂O₂/cat	+	-	-	-	+
H₂O₂/Cu/asc	+	+	0	+	+
ONOOH	+	+	+	+	+
ONOO^-/CO₂	+	+	+	+	+
NO^·	-	-	0	+	-
NO₂^·	+	+	+	+	0
HOCl	+	+	+	+	-

Reaction conditions for each oxidant defined in the text

forms a unique spectroscopic product (2-OH-E⁺) with O₂^·-. The symbol “0” is meant to indicate detection of a very minor reaction with nonphysiologically high concentrations of oxidant; “-” indicates no detectable reaction.