Figure 2.

Variable expression of γ-secretase-cleaved, active Notch1 (Val 1744-NICD) in CRC cells. 100 μg of total cell RIPA lysates were analysed by immunoblotting using anti-Val1744-NICD antibody. Data from 16 cell lines are shown as an example (upper panel). Lysates were also analysed with anti-Hes1 to determine the expression of this well known Notch1 primary target (second panel from top). A short exposure of a western blot with an antibody directed against the C-terminus of Notch1 reveals that the Val1744-NICD fragment is not always the most prominent fragment present in CRC cells (third panel from top; see for example HT55 cells). Actin was analysed as a loading control (bottom panel).