Table 1.
Effects of γ-secretase inhibitors and cisplatin on a panel of CRC cell lines
| CRC line | Val1744-NICD | DAPT 10 μM | L-685,458 5 μM | DBZ 300 nM | Cisplatin cell death (20 μM,48 h) | CP+DBZ combi effect CD (48 h) |
| C80 | + | - | - | - | + | + |
| Caco-2 | + | - | - | - | + | + |
| CCK-81 | + | - | CD | - | (+) | (+) |
| CC07 | + | - | - | - | + | + |
| HCA-7 | + (N) | - | - | - | + | ++(24 h) |
| HCA-46 | + (N) | - | - | - | (+) | + |
| HDC-9 | - | - | - | - | + | - |
| HDC-57 | - | - | - | - | - | - |
| HDC-73 | - | - | - | - | + | - |
| LoVo | + (N) | - | - | - | + | (+) |
| LS123 | + | - | - | - | + | - |
| VACO 4A | + | - | CD | - | - | - |
CD = cell death visible by light microscopy
CP = cisplatin
CP+DBZ = combination treatment with 10 μM CP and 300 nM DBZ
DAPT = N- [N-(3,5-Difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester
DBZ = (S, S)-2- [2-(3,5-Difluorophenyl)acetylamino]-N-(5-methyl-6-oxo-6,7-dihydro-5H-dibenzo [b, d]azepin-7-yl)propionamide
(N) = Val1744-NICD detected in nuclear fraction after cell fractionation
NICD = Notch1 intracellular domain
- (minus symbol) = no visible effect on proliferation, cell death or cell morphology