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Multiparameter FACS analysis of surface-bound, wild-type HRP incubated with l-1 + l-2, d-tyrosinol-A647, and H2O2. The regions outlined by trapezoids schematically represent library sort gates, used to isolate l selective (cells with high A488 and low A647 fluorescence) and d selective (cells with low A488 and high A647 fluorescence) HRP variants.
