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. 2008 Nov 12;105(46):17694–17699. doi: 10.1073/pnas.0809851105

Fig. 4.

Fig. 4.

Enantioselectivities of l selective variants toward 1 and 3 (A) and 2 and 4 (B) as well as d selective variants toward 1 and 3 (C) and 2 and 4 (D), discovered in each round of evolution. Red, yellow, blue, and green bar colors represent substrates 1, 2, 3, and 4, respectively; hatched and solid bars designate surface-bound and soluble HRP, respectively. The l and d letters designate the direction of enantiopreference; the Roman numerals after the letters define the round of directed evolution; the r and/or s letters after the Roman numerals indicate whether these variants were isolated from the random or saturated mutagenesis libraries, respectively. Mutations: LIr (Arg178Gln), LIs (Phe68Leu, Gly69Ala, Asn72Glu, Ser73Leu, Ala74Tyr) (14), LIrs (LIr + LIs), LIIr (LIrs + Gln147Arg), LIII (LIIr + Asn158Asp), DIs (Phe68Glu, Gly69Pro, Asn72Lys) (14), DIIs (DIs + Asn137Arg, Ala140His, Phe142Lys, Phe143Met), and DIII (DIIs + Ser167Ile).