Fig. 3.
Amplification by arachidonic acid of the outsized chemotaxis induced by EDTA. All preparations contain EDTA. (A–C) EDTA control cells, having received only the solvent for the fatty acids (EtOH); D has received oleic acid and E arachidonic acid. (A–C) EDTA control at laser flash (A), 7.6 min later (B), and after a total of 27.6 min (C) when the cell aggregate is at its maximum. (D) EDTA + oleic acid, maximum aggregation, 15.7 min after the laser flash. (E) EDTA + arachidonic acid, maximum aggregation, 32.6 min after the laser flash. EDTA, 10 mM; oleic acid, 250 μM; arachidonic acid, 400 μM; EtOH, 0.2%.