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UBZ mutant hPolη proteins accumulate in replication foci. MRC5 cells were cotransfected with WT GFP PCNA and UBZ mutant FLAG Polη as indicated. Twenty-four hours after transfection, cells were UV-irradiated with 40 J/m2 followed by incubation for 6 h before fixation. FLAG-tagged proteins were immunostained with anti-FLAG mAb. For the accumulation of WT hPolη, see Fig. 3. (Magnification: 600×.)
