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Glycosyl linkage analysis of per-O-methylated LM and ManLAM prepared from M. tuberculosis LM (A), H37RvΔRv2181 LM (B), M. tuberculosis ManLAM (C), and H37RvΔRv2181 ManLAM (D). Samples of purified LM and ManLAM were prepared as described in Materials and Methods, per-O-methylated, hydrolyzed, and reduced, and the per-O-acetylated glycosyl derivatives were analyzed by GC/MS.
