Fig. 3.

Distribution of rupture forces (black line) as a function of the contact time. (A and B) Extension of the contact times shifts the forces toward higher values, indicating binding of >1 receptor molecule. Sums of Gaussians were fitted to the measured curves, yielding an almost perfect reproduction of the experimental data. (C) The binding probability is plotted as a function of the contact time. The solid line is the result of a least-squares fit of a monoexponential decay (0.33 exp((t − 15)/24)).