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Alanine replacement mutagenesis of the PA3455 C-terminal PPK2 domain (A) and SMc02148 (B). Enzymatic activity of purified mutant proteins was measured as polyP-dependent phosphorylation of AMP to ADP (for the C-terminal PPK2 domain of PA3455, residues 261–496) or ADP to ATP (for SMc02148). Experimental conditions were as described in Materials and Methods.
