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. 2008 Nov 18;14:2087–2096.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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rAAV-GFP transduction of organ-cultured human normal and diabetic corneas. Virus serotypes 1, 2, and 8 were used. Upper row – staining with mouse a-GFP MAB3580 (right panel – staining of an organ-cultured cornea not exposed to rAAV-GFP [negative control] with the same antibody [mouse a-GFP]); lower row – staining with rabbit a-GFP antibody ab6662 (right panel – staining of another negative control cornea with antibody ab6662 [rabbit a-GFP]). Epithelial (e), stromal (s), and endothelial (inset panels) cells are well transduced by rAAV-GFP. No staining is seen in corneas not exposed to the GFP-expressing virus. Both antibodies give very similar patterns. GFP was revealed by indirect immunofluorescent staining. DM, Descemet’s membrane. Bar=40 μm.