Figure 5. A3G inhibits the accumulation of longer reverse transcription products more than shorter ones.

(A) Schematic to show how the length of the reverse transcript was measured for each primer/probe set. The exact positions of the primers and probe are not depicted. (B) Natural endogenous reverse transcription reactions were performed for 2 h with HIV-1/Δvif produced in the presence or absence of A3G. Quantitative PCR was carried out with different primer/probe sets to measure the amount of viral cDNA of the various lengths indicated. The amount of cDNA in virions with A3G is plotted as a percentage of that in virions without A3G. Data from Figure 4 are included to show the relative amount of reverse transcripts of 1 base in length. Each point represents an independent pair of viral preparations. Lines indicate the mean value.