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. 2000 Apr 25;97(10):5445–5449. doi: 10.1073/pnas.090087497

Figure 1.

Figure 1

Abnormal patterns of allelic expression detectable in 11p15 imprinted genes in human HCCs. Gain (GOI), loss (LOI), and inversion (INV) of imprinting are shown for normal liver (N) and HCC (T). Corresponding constitutive heterozygous HCC genomic DNA (g) is shown. Analysis of β-actin served to normalize expression levels among matched normal liver and HCC samples. GOI, the most frequently detected abnormality, was considered positive when reduction of expression of one allele in HCC was greater than 70% as compared with the same allele in the matched normal liver; reduction between 25 and 70% was considered partial GOI, and a difference in allele expression less than 25% was considered negative for abnormality. When the imprinted gene was monoallelically expressed in normal liver, GOI led to complete loss of expression in matched HCC (most frequently encountered for CDKN1C). When the gene was biallelically expressed in normal liver, GOI in HCC was detected as a reduction or disappearance of one of the alleles (most frequently observed for the IGF2 gene).