Table 1.
Quantitative analysis of allele specific expression of 11p15 imprinted genes in HCC and matched normal liver samples
| Patient |
SLC22A1L
|
CDKN1C
|
IGF2
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Li | HCC | Ratio | Li | HCC | Ratio | Li | HCC | Ratio | |
| HCC 236 | 100/100 | 100/100 | Normal | 100/15 | 100/40 | Normal | 100/100 | 100/100 | Normal |
| HCC 240 | 100/100 | 100/100 | Normal | / | / | nd | / | / | nd |
| HCC 233 | 100/100 | 100/100 | Normal | hom | hom | nd | hom | hom | nd |
| HCC 244 | 100/20 | 20/20 | GOI | hom | hom | nd | hom | hom | nd |
| HCC 247 | 100/100 | 100/100 | Normal | / | / | nd | hom | hom | nd |
| HCC 249 | hom | hom | nd | hom | hom | nd | 100/35 | 30/100 | INV |
| HCC 248 | hom | hom | nd | hom | hom | nd | 100/100 | 100/100 | Normal |
| HCC 241 | 100/90 | 100/25 | GOI part | 100/100 | 100/0 | GOI | 100/30 | 100/0 | GOI part |
| HCC 232 | 100/35 | 100/35 | Normal | 100/30 | 100/30 | Normal | hom | hom | nd |
| HCC 1462 | hom | hom | nd | hom | hom | nd | 100/100 | 100/60 | GOI part |
| HCC 1430 | 100/80 | 100/80 | Normal | 100/7 | 100/15 | Normal | 100/100 | 100/20 | GOI |
| HCC 1431 | 100/75 | 100/75 | Normal | 100/10 | 0/0 | GOI | hom | hom | nd |
| HCC 1432 | hom | hom | nd | 100/15 | 100/10 | Normal | 100/25 | 25/100 | INV |
| HCC 1433 | 100/100 | 100/100 | Normal | hom | hom | nd | 100/100 | 100/100 | Normal |
| HCC 1434 | / | / | nd | hom | hom | nd | 100/80 | 100/100 | Normal |
| HCC 1435 | 100/100 | 100/100 | Normal | 100/10 | 100/100 | LOI | 100/45 | 100/10 | GOI part |
| HCC 1439 | 100/35 | 30/30 | GOI | / | / | nd | hom | hom | nd |
| HCC 1440 | 100/90 | 100/90 | Normal | 100/20 | 100/20 | Normal | hom | hom | nd |
| HCC 1441 | 100/80 | 100/80 | Normal | hom | hom | LOSS | 100/60 | 100/40 | Normal |
| HCC 1442 | 100/10 | 100/12 | Normal | hom | hom | nd | 100/35 | 35/35 | GOI |
| HCC 1443 | 100/100 | 100/10 | GOI | 100/0 | 0/0 | GOI | hom | hom | nd |
| HCC 1444 | 100/80 | 100/80 | Normal | 100/0 | 45/100 | INV | hom | hom | nd |
| HCC 1446 | / | / | nd | / | / | nd | 100/100 | 100/100 | Normal |
| HCC 1447 | 100/100 | 100/100 | Normal | hom | hom | nd | 100/100 | 100/100 | Normal |
| HCC 1449 | 100/100 | 100/100 | Normal | hom | hom | nd | 100/100 | 100/100 | Normal |
| HCC 1451 | 100/30 | 30/100 | INV | hom | hom | nd | hom | hom | nd |
| HCC 1452 | 100/100 | 100/100 | Normal | 100/12 | 100/15 | Normal | hom | hom | nd |
| HCC 1453 | 100/100 | 100/100 | Normal | / | / | nd | hom | hom | nd |
| HCC 1454 | 100/90 | 100/90 | Normal | hom | hom | LOSS | hom | hom | nd |
| HCC 1456 | hom | hom | nd | 100/20 | 10/10 | GOI | 100/100 | 100/0 | GOI |
| HCC 1457 | 100/80 | 100/80 | Normal | 100/25 | 100/35 | Normal | 100/100 | 100/0 | GOI |
| HCC 1458 | 100/90 | 100/90 | Normal | hom | hom | nd | hom | hom | nd |
| HCC 1459 | 100/100 | 100/100 | Normal | / | / | nd | 100/15 | 100/10 | Normal |
| HCC 1460 | 100/90 | 100/95 | Normal | 100/40 | 100/35 | Normal | hom | hom | nd |
| HCC 1463 | / | / | nd | / | / | nd | 100/80 | 100/0 | GOI |
| HCC 1464 | hom | hom | nd | hom | hom | nd | 100/100 | 100/0 | GOI |
| HCC 1466 | hom | hom | nd | 100/0 | 100/10 | Normal | 100/50 | 100/50 | Normal |
| Total | 52 | 52 | 52 | ||||||
| Analyzed | 46 | 33 | 44 | ||||||
| Heterozygous | 27 | 15 | 21 | ||||||
| Homozygous | 19 | 18 | 23 | ||||||
| Imprinted | 5/27 | 14/15 | 8/21 | ||||||
| GOI | 4/27 | 4/15 | 9/21 | ||||||
| INV | 1/27 | 1/15 | 2/21 | ||||||
| LOI | 0/27 | 1/15 | 0/21 | ||||||
GOI, gain of imprinting; GOI part, partial gain of imprinting; LOI, loss of imprinting; INV, allele inversion; LOSS, loss of expression; hom, homozygous; nd, not determined; Li, normal liver; HCC, hepatocarcinoma.