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. 2008 Aug 26;295(5):E1056–E1064. doi: 10.1152/ajpendo.90345.2008

Fig. 5.

Fig. 5.

Effect of recombinant ANGPTL4 on capillary sprout and tube formation. A: effect of ANGPTL4 on capillary sprout formation from aorta explants. Approximately 7 rings were analyzed per experiment per experimental condition, and the experiment was repeated 3 times. One-way ANOVA followed by Tukey's multiple comparison test yielded P < 0.05 for EGM-2 vs. EGM-2 + 0.5 μg/ml ANGPTL4 and for EGM-2 vs. EGM-2 + 5 μg/ml ANGPTL4. VEGFA and ANGPTL4 induced HUVEC proliferation (B) and capillary tube formation (C) in the absence or presence (+R) of 1 μM RSG. B: shown are the mean ± SE of 3 independent experiments performed in triplicate (total, n = 9). C: 4 fields of each of 3 wells/condition were analyzed, and the experiment was repeated 3 times (total, n = 36). Comparison between experimental groups was performed by Tukey's multiple comparison tests. *P < 0.05; **P < 0.01; ns, Not significant. D: representative photomicrographs of capillary tube formation by HUVEC on Matrigel after 24-h incubation in the presence of the factors indicated. Original magnification is ×40.