Fig. 10.

Downregulation of lipid raft-associated proteins increases internalization of β₁-integrin. A: Western blot analysis of SK-CO15 cells transfected with flotillin-1, flotillin-2, caveolin-1, and Lamin A/C siRNA (control). Note efficient downregulation of all targeted proteins. B: surface biotinylation and β₁-integrin internalization assay in control and caveolin-1-, flotilin-1-, and flotilin-2-depleted SK-CO15 cells. Note a significant increase of β₁-integrin in caveolin-1- and flotilin-2-deficient cells. Data are expressed as a percentage of internalization in Lamin A/C control and are shown as means ± SE, n = 4. *Statistically significant difference compared with control (P < 0.05).