Table 1.

LPS treatment does not increase apelin expression in nonimmune cells

	Primary Cultured Rat Ileal Cells	Enteroendocrine STC-1	Colon* HCT116	Liver HepG2	Breast Hs578T	Capillary Endothelial TR-iBRB2	Hypothalamic GT1-7
Control	3.6±0.2	1.0±0.2	1.0±0.1	1.6±0.1	121.7±6.3	1.2±0.1	0.8±0.1
LPS treated	3.7±0.2	1.0±0.3	1.0±0.3	1.7±0.1	133.2±14.9	1.5±0.1	0.7±0.03

Values are apelin expression levels for the cell types listed, normalized to 18S ribosomal RNA expression levels. LPS dose for primary rat ileal, STC-1, HCT116 cells = 1 μg/ml; LPS dose for HepG2, Hs578T, TR-iBRB2, GT1-7 cells = 200 ng/ml.

^*Other colon cell lines (HT29, KM20, Caco2, and NCM356) gave similar results.