Table 1.
Primary Cultured Rat Ileal Cells | Enteroendocrine STC-1 | Colon* HCT116 | Liver HepG2 | Breast Hs578T | Capillary Endothelial TR-iBRB2 | Hypothalamic GT1-7 | |
---|---|---|---|---|---|---|---|
Control | 3.6±0.2 | 1.0±0.2 | 1.0±0.1 | 1.6±0.1 | 121.7±6.3 | 1.2±0.1 | 0.8±0.1 |
LPS treated | 3.7±0.2 | 1.0±0.3 | 1.0±0.3 | 1.7±0.1 | 133.2±14.9 | 1.5±0.1 | 0.7±0.03 |
Values are apelin expression levels for the cell types listed, normalized to 18S ribosomal RNA expression levels. LPS dose for primary rat ileal, STC-1, HCT116 cells = 1 μg/ml; LPS dose for HepG2, Hs578T, TR-iBRB2, GT1-7 cells = 200 ng/ml.
Other colon cell lines (HT29, KM20, Caco2, and NCM356) gave similar results.