. 2008 Sep 4;105(5):1576–1584. doi: 10.1152/japplphysiol.90670.2008

Table 1.

PCR primers used in the study

Gene, Accession Number	Primer	Sequence (5′–3′)
ChAT, XM_001061520	Sense, external/internal	`GAAGCAGAAATACAGCCCCG`
	Antisense, external	`CACGGGTCCATAACAGCAGA`
	Antisense, internal	`GCAGCCTTGTGGTCAGTCAT`
M1–M3 receptors (degenerate)	Sense, external	`GCCTGTGCWGACCTSATCAT`
	Antisense, external	`CAGAASARRATGGCWGGRGC`
M1 receptor (specific), NM_080773	Sense, internal	`CCATGAACCTCTATACCACG`
	Antisense, internal	`CGAGAAGTAACGGTCAAAGC`
M2 receptor (specific), NM_031016	Sense, internal	`CTACACTGTGATTGGCTACTGG`
	Antisense, internal	`AGAGGATGAAGGAAAGGACC`
M3 receptor (specific), NM_012527	Sense, internal	`TGGGCACTGGGGAACTTAGC`
	Antisense, internal	`GCCAGACCAATCATCACACC`
M4–M5 receptors (degenerate)	Sense, external	`GCCATTGCTGCCTTCTACMT`
	Antisense, external	`GGGTTGATGGTGCTGTTGAC`
M4 receptor (specific), NM_031547	Sense, internal	`TGCCACCCAGAACACCAAGG`
	Antisense, internal	`AGCAGAGCCAGTAGCCGATG`
M5 receptor (specific), NM_017362	Sense, internal	`AGCCAAGAAGAGAGAGCCAG`
	Antisense, internal	`AGCCAGTAACCCAAGTGCCA`
α₄-Subunit of nicotinic receptor, NM_024354	Sense, external/internal	`ATCGTGCCTCGCCTCCTCTT`
	Antisense, external	`TTTGGTGCCTCCCGCCTTGA`
	Antisense, internal	`ACTGCTGTGTCCGTGGGGTT`
Neuron-specific enolase, M11931	Sense, external	`CTCAAGGGGGTCATCAAGGA`
	Antisense, external/internal	`GCTTGGATGGCTTCTGTGAC`
	Sense, internal	`CAAGGCTGGCTACACGGAAA`

ChAT, choline acetyltransferase.