Fig. 3.

Thyrotropin-releasing hormone (TRH)-positive neurons in the PVN do not express FosB. A: immunofluorescence demonstrates RH-induced FosB expression (red) as observed in Figs 1 and 2. B: α-TRH antibody (green) identifies a subpopulation of neurons in the parvocellular PVN and labels both cell bodies and fibers. C and D: successively higher (×40 and ×60) magnification merged images, respectively, of the same field double-labeled for both FosB and TRH; no colocalization is observable. Scale bar represents 100 (A and B); 50 (C), and 25 (D) μm.