Skip to main content
. 2008 Aug 27;295(5):R1446–R1454. doi: 10.1152/ajpregu.90511.2008

Fig. 5.

Fig. 5.

Glutamic acid decarboxylase (GAD) immunoreactivity, a marker of GABAergic transmission, does not colocalize with FosB in the PVN following RH. Immunofluorescence using α-GAD67 antibody revealed intense GAD (green) expression in the hypothalamus. A: GAD-positive staining in the PVN and peri-PVN. Note that the staining is almost exclusively of fibers within the PVN proper (outlined by dotted white line), whereas GAD-positive immunoreactive cell bodies are only observed in the area of the “peri-PVN” (white arrows). B: FosB-positive cells (red) in the PVN following RH. C: GAD immunoreactivity in the PVN and surrounding area in the same section. The neuropil in the immediate region of the PVN displayed intense GAD-positive fiber staining. D: merged images of the same field illustrating the intense GAD-positive neuropil with contacts appearing to impinge on FosB-positive cells. Scale bar: 100 μm.